Visionneuse

Date_TXT

Berlin : Berlin , 2002

Cote

597 RAU

Auteur

Rautenstraub, Bernd W.

Sujet

Poissons ; Technologie ; Chromosomes

Type de document

Livre

Description :

Fluorescence in situ hybridization (FISH) is a powerful method to directly visualize the localization of genomic alterations in the nucleuses: The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetic, and biology.
Topics described in this manual include:
-FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells;
-FISH on archival human tissues, e.g. formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH);
-FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION);
- FISH related techniques, such as PRINS arid microarrays.